There are basically two groups of human characters on the island, the Endurance survivors and the scavengers.
- Main article: Endurance#Crew
- Lara Croft
- Conrad Roth - Captain of the Endurance
- Samantha Nishimura - A female crewmember of the Endurance and Lara's close friend. Taken hostage by the scavengers early on.
- Dr. Whitman - A scientist on board the Endurance, strangely fascinated with the scavengers culture.
- Alex Weiss - Endurance's technician
- Angus Grimaldi - Helmsman and first aid officer of the Endurance
- Jonah Maiava - Ship's cook
- Joslin Reyes - The mechanic of the ship
- Mathias - A scavenger who pretends to be not one of the group, he takes Sam captive.
- Vlad - A Russian scavenger who assaults Lara and becomes her first kill.